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ANALISIS MOLECULAR EN GASTROPODOS CHILENOS COMERCIALES BASADOS EN LAS SECUENCIAS 16S rRNA,. COI Y ITS1-5.8S rDNA-ITS2 Gayana
Aguilera-Muñoz,Felipe; Lafarga-Cruz,Fabiola; Gallardo-Escárate,Cristian.
Gastropod mollusks are part of the principal marine resources cultivated and commercialized in Chile. There are native Chilean species such as loco (Concholepas concholepas), locate (Thais chocolata), trumulco snail (Chorus giganteus), keyhole limpets (Fissurella spp.), tegula snail (Tegula atra) as well as exotic species such as red abalone (Haliotis rufescens) and Japanese abalone (Haliotis discus hannai). Despite their importance as marine resources, molecular genetic studies establishing phylogenetic relationships and estimating population genetic parameters are scarce. The aim of this study is to establish a molecular approach among the main commercial gastropod species in Chile. The mitochondrial genes 16S rRNA and COI, and the nuclear ribosomal...
Tipo: Journal article Palavras-chave: 16S rRNA; COI; ITSl-5.8SrDNA-ITS2; Gastropod mollusks; Phylogenetic relationships.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382009000100003
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AUTHENTICATION OF COMMERCIAL CHILEAN MOLLUSKS USING RIBOSOMAL INTERNAL TRANSCRIBED SPACER (ITS) AS SPECIE-SPECIFIC DNA MARKER Gayana
Aguilera-Muñoz,Felipe; Valenzuela-Muñoz,Valentina; Gallardo-Escárate,Cristian.
Contemporary world food safety standards have emphasized the implementation of efficient food traceability systems, including the correct labeling of products for the authentication of the commercialized species. In this context, Chile has reached an important development in agricultural exportations, reaching near US$ 3.82 billion in 2007. Since the identification based on morphologic and organoleptic characteristics is complex during the elaboration process of aquaculture products, molecular tools need to be developed in order to genetically trace aquaculture products. This study analyzed five species of Chilean commercial mo Husks using molecular DNA markers. From the partial sequences of the ribosomal region ITSl-5.8SrDNA-ITS2, the specific primers for...
Tipo: Journal article Palavras-chave: Genetic traceability; ITS 1; ITS2; Mo Husk identification; Specific divider design.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382008000200007
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